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The immuno-stimulatory effect of mRNA produced through chaotropic agent-included IVT was evaluated using a THP1-Dual Monocyte cell line. All authors are employees of BioNTech RNA Pharmaceuticals, a company that develops mRNA-based therapies. Like DNA replication, there are three stages to transcription: initiation, elongation, and termination. 26 Transcription: from DNA to mRNA In the first of the two stages of making protein from DNA, a gene on the DNA molecule is transcribed into a complementary mRNA molecule. Finally, the mRNA is degraded. Messenger (m)RNA vaccines require a safe and potent immunostimulatory adjuvant. In this study, we introduced immunostimulatory properties directly into mRNA molecules by hybridizing them with complementary RNA to create highly immunogenic double stranded (ds)RNAs. Overview of the Stages of Transcription. The basic steps of transcription are initiation, elongation, and termination. Here we can identify several of the DNA sequences that characterize a gene. The promoter is the binding site for RNA polymerase. Next Generation Sequencing (NGS) is driving rapid advancement in biological understanding and RNA-sequencing (RNA-seq) has become an indispensable tool for biology and medicine. There is a growing need for access to these technologies although preparation of NGS libraries remains a bottleneck to wider adoption
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